| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d75d1a4caed60a9316608ed9c231546c |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
| filingDate |
2003-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af112ec8794129f6b22784fe5ba5e24a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_663f9aeadc49865cf3aedfc5c441835a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0abfb3ecfee1a31ed53a2e3fc992f19e |
| publicationDate |
2005-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2005098830-A |
| titleOfInvention |
Screening method for protein interaction substances by mass spectrometry |
| abstract |
【Task】 Providing a method for efficiently screening substances that interact with proteins by combining pull-down assay methods using affinity beads and mass spectrometry as the first step in starting functional analysis of proteins. [Solution] (A) performing mass spectrometry of two or more candidate compounds and measuring the ionization efficiency of each candidate compound; (b) contacting the target protein with a mixture containing the two or more candidate compounds; ) Removing a candidate compound that does not bind to the target protein (non-binding), (d) recovering a complex of the target protein and the candidate compound, (e) performing mass analysis of the complex, and A step of measuring the target protein contained in the body and the ionization amount of each candidate compound, and (f) the amount of each candidate compound contained in the complex or the ratio thereof based on the results of steps (a) and (e) A ligand compound that binds to the target protein is identified. [Selection] FIG. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-112010000814-T5
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010096642-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2010041459-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010175467-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010540947-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010041459-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-112010000814-B4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114441663-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007254397-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4486166-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8865418-B2 |
| priorityDate |
2003-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |