http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114441663-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e5c4932e4b04980ccfa1e2cd608246e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-062 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 |
| filingDate | 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1855bc8b34881a4881b21ec0f7791b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc7c7b83774e84ba4d75b2312208872a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d59d2ccd52fcf54a37763198e9df7ff3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3c0d93dc70159817e2b0611542777e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7cc65bf58d3259add6320b7f0165ef66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31c04c3a823c7506767c5a046a05fdc8 |
| publicationDate | 2022-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114441663-A |
| titleOfInvention | A method for screening protein-positive compounds using solid-phase microextraction affinity-selective mass spectrometry |
| abstract | The present invention relates to a method for screening protein-positive compounds using solid-phase microextraction affinity-selective mass spectrometry. The method uses a micropipette tip with a filter frit as a reactor, which is filled with a solid-phase matrix, and the target protein passes through it. The affinity purification tag sequence contained in the protein sequence is bound to the surface of the solid phase matrix, and positive compounds are retained on the surface of the solid phase matrix by binding to the target protein, and then the unbound compounds are separated and eluted by centrifugation, so as to be specific Positive compounds were enriched. Compared with the traditional method, the reaction system of this method is smaller and the non-specific adsorption is low; the solid-phase matrix is retained by the sieve plate, which effectively avoids the loss of the solid-phase matrix during the centrifugal separation process, and ensures that this method has better experimental parallelism and screening. accuracy. In addition, the method takes very short time for each solid phase separation, and has strong operability and high convenience. |
| priorityDate | 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.