| abstract |
PROBLEM TO BE SOLVED: To provide a nucleic acid measuring method using a nucleic acid probe labeled with a fluorescent dye, and real-time quantitative PCR using the method And a method for analyzing data obtained by the PCR method in a short time and accurately. SOLUTION: A nucleic acid probe labeled with a fluorescent dye, or a nucleic acid probe consisting of a 2-O-methyl oligonucleotide, a chimeric oligonucleotide or the like or an oligonucleotide interposed therebetween is hybridized to a target nucleic acid. , A novel nucleic acid measurement method for measuring the amount of decrease in luminescence of a fluorescent dye before and after hybridization, real-time quantitative PCR using the method, and fluorescence during an annealing reaction when analyzing data obtained by the PCR method Provided is a data analysis method including a step of correcting an intensity value with a value at the time of a denaturation reaction. |