abstract |
(57) [Summary] [PROBLEMS] To provide a method for simply and accurately quantifying hemoglobin A1c (HbA1c) in a sample. SOLUTION: A test sample is prepared by using β chain N of hemoglobin A1c. Protease capable of cleaving the carboxyl group side of the third leucine from the terminal, then H from fructosyl-Val-His-Leu A method for quantifying hemoglobin A1c, which comprises sequentially treating is-Leu with a protease capable of cutting out and then measuring fructosyl valine generated there. |