| abstract |
The invention is directed to a method for detecting a target moiety in a sample of biological specimens by: na) providing at least one conjugate with the general formula (I) X n - P - Y m , with X is an detection moiety, P an enzymatically degradable spacer and Y an antigen recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P, nb) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y nc) detecting the target moiety labeled with the conjugate with the detecting moiety X and nd) enzymatically degrading spacer P, thereby cleaving the detection moiety X from the conjugate. n The method is useful to identify target moieties on the biological specimens. The biological specimens detected by the conjugate can be subsequently removed from the sample. |