| abstract |
A recombinant DNA transfer vector contains a leader sequence polynucleotide which codes for a signal polypeptide of formula 1, n Preferably the transfer vector is a plasmid. In one preferred embodiment the leader sequence polynucleotide is downstream of and in reading phase with a bacterial or yeast promotorand a ribosome binding site, and upstream of and in reading phase with a structural gene. The structural gene may be, for example, the carboxypeptidase G 2 (CPG 2 ) gene from the chromosomal DNA of Pseudomonas species strain RS - 16. Examples of plasmids containing the leader sequence polynucleotide and the CPG 2 gene are pNM1, pNM111, pNM14, pNM21, pNM22, pNM31, pNM32 and pLEC3. |