http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250205-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d3050f1fcbf433ef34335490698a56 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01159 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
| filingDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9922dfca5cc612e08e886d43071c3ef |
| publicationDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114250205-A |
| titleOfInvention | A 7α-hydroxysteroid dehydrogenase mutant with high thermal stability and its application |
| abstract | The invention discloses a 7α-hydroxysteroid dehydrogenase mutant with high thermal stability, belonging to the technical field of biological enzymes. The invention changes the hydrophobicity of the molecular surface by mutating the 108th aspartic acid of the amino acid sequence of the wild-type 7α-hydroxysteroid dehydrogenase into a more non-polar isoleucine, without affecting the activity. The thermal stability of 7α-hydroxysteroid dehydrogenase has been improved, the activity time of the enzyme in the reaction system with higher temperature is prolonged, and the maintenance time of high activity of the enzyme at high temperature is avoided, and the activity of the enzyme is low when the temperature is low. The embarrassing situation of a long time can effectively solve the problem that industrial production control low temperature requires more energy and is difficult to achieve, and is suitable for industrial large-scale production. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114231508-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114231508-A |
| priorityDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 211.