http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114774380-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d3050f1fcbf433ef34335490698a56 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01159 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01201 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9922dfca5cc612e08e886d43071c3ef |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114774380-A |
| titleOfInvention | A kind of expression method of highly active hydroxysteroid dehydrogenase |
| abstract | The invention discloses an expression method of a highly active hydroxysteroid dehydrogenase, which belongs to the technical field of biological enzymes. The expression method comprises the following steps: using plasmid pET 32a as a starting vector, constructing a recombinant expression plasmid containing the hydroxysteroid dehydrogenase gene, introducing the constructed recombinant expression plasmid into a host cell, culturing the host cell to express the hydroxysteroid dehydrogenase gene, wherein the host cell is Origami2 (DE3). In the present invention, pET32a is used as the expression vector of 7α-HSDH and 7β-HSDH, and Origami2 (DE3) is used as host bacteria, which can effectively improve the expression amount and activity of hydroxysteroid dehydrogenases such as 7α-HSDH and 7β-HSDH. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250205-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250205-B |
| priorityDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.