| abstract |
The invention relates to the technical field of genetic engineering and protein transformation, and discloses a heat-salt-sensitive inulinase mutant MutY119D and a preparation method thereof. The amino acid (tyrosine) at position 1 was obtained by mutating the amino acid (tyrosine) to aspartic acid, and the sequence of MutY119D is shown in SEQ ID NO.1. Compared with the wild enzyme InuAMN8, the mutant enzyme MutY119D has improved low-temperature activity, reduced thermal stability, and reduced activity and stability in sodium chloride, which is beneficial to the safe use of the enzyme and its application in the field of biotechnology under low-temperature environment requirements. . The thermosalt-sensitive inulinase mutant MutY119D of the present invention can be applied to industries such as food, brewing and washing. |