http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103074451-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12730c7008616a2d5145d4f153352422 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-445 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
filingDate | 2013-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa34a01fe4f4c62762094191d5cabbf3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02fad26f1755b397577755f7eb9416df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79d4127ee1b13cc2a1fed6b5276f1f87 |
publicationDate | 2014-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103074451-B |
titleOfInvention | Kit for synchronously detecting twenty-two respiratory tract pathogens and detection method of kit |
abstract | The invention discloses a kit for synchronously detecting twenty-two respiratory tract pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine respiratory tract pathogens, and a reverse primer of a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-6 (sequence identifier number 1-6), and the PCR primer comprises forward and reverse PCR amplification primers of the rest thirteen respiratory tract pathogens, a forward and reverse amplification primer of a human DNA internal reference, a forward and reverse PCR amplification primer of a reaction internal reference, PCR amplification primers of the nine respiratory tract pathogens, and a PCR amplification primer of the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 7-44. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106676174-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106701953-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106591468-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106591468-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106701953-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106676174-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108866071-A |
priorityDate | 2013-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.