http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106591468-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_64c08dbbd66b52c9a43aa3682ae4aafe |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2017-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89d1c050758e354ab4c1ba5a29dafd06 |
| publicationDate | 2017-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106591468-A |
| titleOfInvention | Primer composition, kit and method for detecting the expression level of key genes of autophagy in Drosophila melanogaster |
| abstract | The invention discloses a primer composition, a kit and a use method for detecting the expression level of key autophagy genes in Drosophila melanogaster. The primer composition of the present invention includes RT amplification primers and PCR amplification primers for 10 key genes of autophagy and RNA internal reference. The kit includes DEPC water, 5×RT buffer, reverse transcription primer, reverse transcriptase, Z solution, 10×PCR buffer, PCR primer, 25mM magnesium chloride solution, DNA polymerase and positive control. The invention can detect 10 key autophagy genes of Drosophila melanogaster at the same time, and can complete the detection of 192 samples in one day, which not only saves production and detection costs, but also improves detection efficiency; the RNA internal reference provides a control for the integrity of the sample RNA The internal reference ensures the judgment of sample quality during the inspection process, avoids false negatives, and makes the detection have better sensitivity and specificity, thereby avoiding the problem of low specificity of other detection methods. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110790833-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110790833-A |
| priorityDate | 2017-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 173.