http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106676174-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_64c08dbbd66b52c9a43aa3682ae4aafe |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2017-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89d1c050758e354ab4c1ba5a29dafd06 |
| publicationDate | 2017-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106676174-A |
| titleOfInvention | Primer composition, kit and method for detecting the expression level of insulin-like peptide ILP series genes in Drosophila melanogaster |
| abstract | The invention discloses a primer composition, a kit and a use method for detecting the expression level of insulin-like peptide ILP series genes in Drosophila melanogaster. The primer composition comprises RT amplification primers and PCR amplification primers of insulin-like peptide and RNA internal reference. The kit includes DEPC water, 5×RT buffer, reverse transcription primer, reverse transcriptase, Z solution, 10×PCR buffer, PCR primer, 25mM magnesium chloride solution, DNA polymerase and positive control. The present invention can detect 7 insulin-like peptides of Drosophila melanogaster at the same time, and can complete the detection of 192 samples in one day, which not only saves production and detection costs, but also improves detection efficiency; the RNA internal reference provides a control internal reference for the integrity of the sample RNA, Ensure the judgment of sample quality during the inspection process, avoid false negatives, and make the detection have better sensitivity and specificity, thereby avoiding the problem of low specificity of other detection methods. |
| priorityDate | 2017-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 258.