http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9958725-A1

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate 1998-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8ddb36de81aba6b74e3e5106a0c15a6
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publicationDate 1999-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9958725-A1
titleOfInvention Process for obtaining amplified fragments of nucleic acid of viral and sub-viral particles which contain rna
abstract The invention relates to a process which comprises capturing on a tube of a thermocycler the viral or sub-viral particles, adding into the tube the reagents which are necessary to carry out the reverse transcription (RT) and the enzymatic amplification by polymerase chain reaction (PCR), introducing the tube into the thermocycler and applying a thermocycle appropriate to effect first the reverse transcription and then the PCR without having to add reagents between both reactions and without having to manipulate the tubes with samples. To this effect, a thermostable DNA polymerase is used in the PCR. The amplified fragments can be subjected to various analyses for detecting, identifying and typing the viral or sub-viral particle. This process can be applied to diagnosis and studies of molecular epidemiology.
priorityDate 1996-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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Total number of triples: 29.