| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1994-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01b36f74c1ee687a6773f139013ee050
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf211b0e73ff9694e53aa5a55297fc4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d67ab36360f9bdde209e6ac5c000bfa4 |
| publicationDate |
1995-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0632134-A2 |
| titleOfInvention |
Reagents and methods for coupled high temperature reverse transcription and polymerase chain reaction |
| abstract |
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. The reverse transcription reaction is performed in an appropriate buffer comprising a metal buffer which buffers the divalent cation concentration and which buffer preferably buffers both the pH and the divalent cation concentration. Said divalent cation is preferably Mn²⁺. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents and kits particularly suited for the methods of the present invention are provided. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0736609-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1594982-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2206790-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5981183-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0736609-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1594982-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6916616-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6303306-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9632501-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6124093-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10378050-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9735988-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9958725-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9821362-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9821362-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0776981-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2121698-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5916779-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11085042-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03048392-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9127308-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10094800-B2 |
| priorityDate |
1993-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |