http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9903326-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cea139ad438b9ce7d33159d45b154c6b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8231 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 |
| filingDate | 1998-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_049335584ff0f37ee952d1a1715e7ed6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_204d7b3f62868f64b3bbec2dd32634ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a954bcccfc23c390e70204157bfdd9f0 |
| publicationDate | 1999-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9903326-A1 |
| titleOfInvention | Pollen-based transformation system using solid media |
| abstract | A medium is disclosed that supports pollen germination and pollen tube growth in the presence of Agrobacterium, the medium comprising agarose, sucrose, NO3, MnSO4, H3BO3, MgSO4 and gibberellic acid. A method is disclosed for the genetic transformation of plants and lines by a pollen-based Agrobacterim-mediated transformation. The method comprises the steps of obtaining pollen from a first plant, applying a lawn of Agrobacteria to a solid pollen culture medium, the Agrobacterium comprising at least one heterologous gene sequence capable of being transferred to a plant cell, applying the pollen to the solid medium, allowing the pollen to germinate and grow on the medium, whereby the Agrobacterium attaches to the pollen tube or mediates transfer of the heterologous gene sequence to the germinating pollen to obtain transgenic pollen, applying the transgenic pollen to the stigma of a second plant capable of being fertilized by the pollen of the first plant, thereby fertilizing the second plant, obtaining transgenic seed from the second plant and germinating the transgenic seed to obtain a transgenic plant. The method is particularly suited for use with so-called 'dry stigma' pollen. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2302627-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104004702-A |
| priorityDate | 1997-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.