http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9838337-A1

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filingDate 1998-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9599ac43b874871bf69957eb3e714398
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publicationDate 1998-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9838337-A1
titleOfInvention Methods for cleaving dna with nucleotide integrases
abstract The present invention provides methods, using a nucleotide integrase, for cleaving nucleic acids substrates at specific sites and inserting a nucleic acid molecule into the cleaved substrate. The method of cleaving one strand of a double-stranded DNA substrate comprises providing a nucleotide integrase comprising a group II-intron RNA having two hybridizing sequences capable of hybridizing with two intron RNA binding sequences on the one strand of the substrate and a group II-intron encoded protein which binds to a first sequence element of the substrate. The method of cleaving both strands of a double-stranded DNA substrate comprises providing a nucleotide integrase comprising a group II-intron RNA having two hybridizing sequences capable of hybridizing with two intron RNA binding sequences on one strand of the substrate and a group II-intron encoded protein capable of binding to first and second sequence elements in the recognition site of the substrate. The method of cleaving a single-stranded nucleic acid substrate comprises providing an integrase having two hybridizing sequences capable of hybridizing with two intron RNA-binding sequences of the substrate and a group II-intron encoded protein.
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priorityDate 1997-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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