| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efaece3f57d915a44fb0784b21caeab8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate |
1995-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1997-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80129f4c83baabb947a3c76de142ed10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14f90b20d40b8d65036b039aa6bc5370
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd1e057b21a35d0175bb3649865602cf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c64001e0fdbe7e37937b2d20c8b6ca4 |
| publicationDate |
1997-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5698421-A |
| titleOfInvention |
Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site |
| abstract |
The present invention provides new methods, employing a nucleotide integrase, for cleaving double-stranded and single stranded DNA substrates at specific sites and for attaching nucleic acid molecules to the cleaved DNA substrates. One method uses a nucleotide integrase to cleave one strand of a double-stranded DNA and to concomitantly attach a nucleic acid molecule to the cleaved strand. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach a nucleic acid molecule to one strand of the DNA substrate. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach an RNA molecule to one strand of the substrate and for attaching a cDNA to the other strand of the substrate. Another method cleaves single stranded DNA with the concomitant insertion of a nucleic acid molecule at the cleavage point. The nucleotide integrase comprises an RNP particle which comprises a group II intron RNA bound to a group II intron encoded protein. The present invention also relates to purified and reconstituted RNP particles and reconstituted RNP that cleave DNA substrates. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007254291-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2511287-A2
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009142838-A1
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006192517-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0129059-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7919322-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004005673-A1
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003104352-A1 |
| priorityDate |
1995-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |