| abstract |
Devices and method are provided for precise reduction of arrays of microspots. A pulse jetting device is employed having a capillary (16) of micron dimensions, with a portion of the capillary proximal to the jetting orifice (60) circumferentially surrounded by a piezoelectric transducer (56). By appropriate design of the capillary (16), orifice (60) and piezoelectric transducer (56), droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject subtrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample. |