| abstract |
In a process for detecting p53-specific antibodies in body fluids, p53 and/or fragments thereof having binding regions for p53-specific antibodies immobilised on a substrate are incubated with body fluids and the specific antibodies (a) bound to p53 and/or its fragments are reacted with labelled antibodies (b) directed against the antibodies (1), or with unlabelled antibodies (b), then the latter are reacted with marked antibodies (c) directed against the antibodies (b). All labelling is non radioactive. Also disclosed are kits for carrying out this process, p53-fragments having binding regions for p53-specific antibodies, the DNA sequences coding for said fragments, as well as a process for producing the same. |