| abstract |
A liquid phase assay kit is disclosed for determining the quantity of a predetermined oligonucleotide of a biological analyte, the assay kit being for use with a first polynucleotide probe and a second polynucleotide probe comprising: a) masked receptor means for attaching the first probe containing a target binding region, the masked receptor means having attachment sites therein; b) ligating means for combining with the first probe, the ligating means, upon attaching to the first probe and contacting the masked receptor, site functioning to attach the first probe to the receptor site; and, c) labeling means for combining with the second probe, the labeling means, upon attaching to the second probe and to the analyte, functioning to provide a quantitatable display in a liquid phase. |