http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1164201-A1

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filingDate 2000-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_955f42e6733649413b0b02f425580a6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac1f0b6c9312ffbe5aff8771faacd717
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publicationDate 2001-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1164201-A1
titleOfInvention Reverse detection for identification and/or quantification of nucleotide target sequences on biochips
abstract The present invention relates to a method fornthe identification and/or the quantification of one or morentarget nucleotide sequences (3) present in a sample andndiscrimination from possibly homologous sequences, by usingntwo sets of nucleotide sequences (1 and 2), characterisednin that the first set of nucleotide sequences possiblynlabelled (1) bind specifically to said target nucleotidensequences (3) in a first step and are detected and/ornquantified through hybridisation with the second of capturennucleotide sequences (2) having at least a part of sequencencomplementary to the possibly labelled nucleotide sequencen(1) of the first set of nucleotide sequences, said capturennucleotide sequence (2) being immobilised upon the surfacen(4) of a solid support according to an array of at least 4ndiscrete regions/cm 2 , each of said discrete regions beingnbonded with one species of capture nucleotide sequencesn(2), and the identification and quantification of thenbinding between the possibly labelled nucleotide sequencen(1) and their corresponding capture nucleotide sequence (2)nis correlated with the identification and thenquantification of a target nucleotide sequence (3) presentnin the sample.
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