abstract |
The invention relates to a gene which pertains to D-N-carbamoyl-$g(a)-amino acid amidohydrolase which converts D-N-carbamoyl-$g(a)-amino acid into D-$g(a)-amino acid; a recombinant plasmid comprising a DNA fragment containing the gene integrated into a vector; a microorganism belonging to the genus $i(Escherichia), $i(Pseudomonas), $i(Flavobacterium), $i(Bacillus), $i(Serratia), $i(Corynebacterium) or $i(Brevibacterium) and transformed by integrating the recombinant plasmid therein; a process for producing D-N-carbamoyl-$g(a)-amino acid amidohydrolase by culturing the transformed microorganism; D-N-carbamoyl-$g(a)-amino acid amidohydrolase produced thereby; and a process for producing D-$g(a)-amino acid by the action of the amidohydrolase. The amidohydrolase can be used also in a state immobilized on a carrier. |