| abstract |
Vectors effective for the expression and secretion of heterologous genes in streptomycetes are disclosed. Such vectors comprises a plasmid replicable in Streptomyces, which comprises a Streptomyces promoter and a DNA sequence encoding a Streptomyces signal sequence operably linked to, and under control of the promoter, which DNA sequence encoding a Streptomyces signal sequence may be operably linked to a heterologous gene encoding a desired protein so that the Streptomyces signal sequence and heterologous protein are expressed, and the desired protein is secreted under control of the Streptomyces signal sequence. Various Streptomyces promoters and secretion signal sequences may be used in the invention, and DNA sequences comprising the promoters operably linked to DNA sequences encoding Streptomyces signal sequences, are disclosed as part of the invention. Vectors comprising the Streptomyces aph, ermE, ermEa1 and modifications thereof, controlling the expression of the amy and ORF438 signal sequences, which lead to secretion of desired heterologous proteins when transformed into Streptomyces hosts, are exemplified. |