http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022227102-A1

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filingDate 2021-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89ae3423feb706b6ee7561f85117bb3f
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publicationDate 2022-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2022227102-A1
titleOfInvention METHOD FOR IMPROVING PLANT STRESS RESISTANCE AND YIELD BY HETEROLOGOUS SYNTHESIS OF γ-POLYGLUTAMIC ACID IN PLANT
abstract Disclosed is a method for improving plant stress resistance and yield by heterologous synthesis of γ-polyglutamic acid in a plant, comprising: cloning, from a strain producing γ-PGA, three key enzyme genes PgsA, PgsB and PgsC for synthesizing the γ-PGA; re-synthesizing, according to codon preference in the plant, codon-optimized PgsA, PgsB and PgsC gene sequences; connecting codon-optimized PgsA, PgsB and PgsC genes to a vector pU130-bar to obtain a plant expression vector PGA001; and performing Agrobacterium tumefaciens-mediated transformation into the plant to obtain a new line of transgenic plant for producing γ-polyglutamic acid. Experiments prove that a new line of transgenic maize obtained by the method can improve not only the drought resistance but also the salt resistance, and can significantly increase the biomass of the maize under drought stress conditions and normal growth conditions. The above-mentioned results indicate that the method of the present invention is a green, efficient and sustainable effective way to achieve stable yield or less yield reduction of crops under drought, water shortage and saline-alkali stress, and can be widely applied to drought-resistant and salt-tolerant molecular breeding and new variety cultivation of plants.
priorityDate 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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