| abstract |
The invention discloses a method for heterologous synthesis of γ-polyglutamic acid in plants to improve plant stress resistance and yield. The three key enzyme genes PgsA, PgsA, PgsB and PgsC, and resynthesize the codon-optimized PgsA, PgsB, and PgsC gene sequences according to the codon preference in plants, and link them into the vector pU130-bar to obtain the plant expression vector PGA001, which is mediated by Agrobacterium into In plants, a new line of transgenic plants producing γ-polyglutamic acid is obtained. Experiments show that the new line of transgenic maize obtained by the method of the present invention can not only improve drought resistance but also salt resistance, and can significantly increase the biomass of maize under drought stress conditions and normal growth conditions. The above results indicate that the method of the present invention is a green, high-efficiency and sustainable solution to stabilize or reduce yield of crops under drought, water shortage and saline-alkali stress. Breeding. |