Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9a66b0bf1cce655ea85ad7221bba215 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
filingDate |
2021-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_755f80171b19ee2183a2667c07a05ebe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64b085cb0215c0c7ec572114ce38aa46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25bccbeae95880a917a61325186dbe21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b2489448bab8c9f8c6ba2ec2076ac2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74b78c669992a2b3e5a641f865046105 |
publicationDate |
2021-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2021225886-A1 |
titleOfInvention |
Compositions and methods for modifying polymerase-nucleic acid complexes |
abstract |
Provided herein include a method for modifying polymerase-nucleic acid complexes, including (a) providing a plurality of surface-immobilized polymerase-nucleic acid complexes in a vessel, wherein the nucleic acid includes a primed-template nucleic acid, wherein at least a subset of the surface-immobilized polymerase-nucleic acid complexes include ternary complexes further including nucleotides; and (b) washing the surface with an aqueous solution including a diol, sulfoxide or polyol, thereby removing the nucleotides from the vessel and retaining the surface-immobilized polymerase-nucleic acid complexes in the vessel. |
priorityDate |
2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |