| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9a66b0bf1cce655ea85ad7221bba215 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-518
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-518
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate |
2017-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23ea1d388173508a8070016ea65469f3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbc2e0ebe9c45e6047977837a33ca648 |
| publicationDate |
2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10584379-B2 |
| titleOfInvention |
Method of nucleic acid sequence determination |
| abstract |
Provided are sequencing-by-binding methods of detecting cognate nucleotides using a crippled DNA polymerizing enzyme that possesses the ability to bind the next correct nucleotide downstream of a primer in a template-dependent fashion, but does not possess the activity needed to promote phosphodiester bond formation. Use of the crippled DNA polymerase permits interrogation of one nucleotide at a time, without incorporation of any nucleotide. Labeled nucleotides, such as fluorescently labeled nucleotides, can be used in conjunction with the crippled DNA polymerase to establish cognate nucleotide identity in a rapid manner. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11287422-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10768173-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023081485-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022240764-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022240766-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021225886-A1 |
| priorityDate |
2016-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |