| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_014b688e1f7db4cc95f7f8d7205c0a44 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-108
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0609
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate |
2021-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c89f8da5169ba2350529820133acdf3a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3aee307d92acfc55c54cb92b1e5a769 |
| publicationDate |
2021-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2021144574-A1 |
| titleOfInvention |
Gene editing of unfertilized porcine and bovine oocytes |
| abstract |
Methods for creating gene edited swine and cattle at industrial quantities are provided. The methods result in reduced rates of mosaicism as compared to traditional gene-editing methods. Animals are synchronized and unfertilized, metaphase II (MII) oocytes, are harvested from the animals (e.g., via flushing or OPU). Gene editing components are introduced into the MII oocytes. For example, the oocytes can be injected with a Cas protein and at least one guide RNA (gRNA) directed to a target gene. Injected oocytes can be incubated prior to fertilization up to the point of viability, cultured to appropriate stages, and then implanted into surrogate females for gestation. Optionally, the superovulated females can be inseminated prior to harvesting the unfertilized oocytes, and zygotes can be gene edited via conventional means while unfertilized oocytes can be gene edited via a method of the present teachings. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023105244-A1 |
| priorityDate |
2020-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |