abstract |
The disclosure relates to methods of transformation and related compositions. In some aspects, the methods comprise wounding at least part of a region of an embryo axis comprising an epicotyl, a shoot apical meristem, and cotyledonary node, or the corresponding region in a monocot embryo axis, to produce a wounded explant, and contacting the wounded explant with a heterologous polynucleotide. In some aspects, the methods further comprise on or more selection steps which may be done in planta . |