http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020129027-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_620d8e0b5ce849d2ace9151c7b23d596 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4738 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-042 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate | 2019-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55cbee09c0f1ad631e80ee1b87fa7b7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f9f54acc97268c94761ba5ed41a8309 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cefce75198c061c688d5b7a17d5b0745 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ec6a7a7ef94b2f43a2abafe4fbbc1e0 |
| publicationDate | 2020-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020129027-A1 |
| titleOfInvention | Sliding clamp-based affinity purification systems, methods of making and use thereof |
| abstract | Described are affinity purification system that includes a carrier/surface that is non-cellular, and sliding clamp (SC) protein, and methods for purifying proteins that bind to the SC. The SC is associated with the carrier/surface via covalent/non-covalent interactions.To attain control of coupling site, the SC can be mutated via site-directed mutagenesis to introduce an exogenous residue and, the exogenous internal residue is conjugated to the non-cellular surface through the linker. The SC can also be coupled to the carrier via non-covalent interactions such as the affinity interactions involved in ligand/binding partner complex formation. The SC-based affinity purification system are used in a purification column as bait proteins, to isolate SC binding partners or non SC-binding proteins engineered to contain a SC binding site prior to its purification. |
| priorityDate | 2018-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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