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filingDate 2019-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019239805-A1
titleOfInvention Digital pcr measurement method and measurement device
abstract The present invention addresses the problem of providing a novel digital PCR analysis method. An embodiment of this novel digital PCR analysis method is a DNA detection method including a step for dividing a DNA solution containing a fluorescent labeling probe or a DNA intercalator and DNA to be detected into a plurality of fractions, a step for performing a nucleic acid amplification reaction in the fractions, a step for measuring fluorescence intensity in conjunction with temperature variation, a step for calculating the melting temperature of a DNA double strand measured on the basis of a variation in the fluorescence intensity accompanying the temperature variation, and a step for calculating the ratio, with respect to the fluorescence intensity at a first temperature accompanying the temperature variation, of the fluorescence intensity at a second temperature lower than the first temperature.
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priorityDate 2018-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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