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filingDate 2019-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019181999-A1
titleOfInvention Method for three-dimensionally culturing pluripotent stem cells
abstract The present invention addresses the problem of providing a method for three-dimensionally culturing pluripotent stem cells whereby good proliferation properties of the pluripotent stem cells can be maintained even though the frequency of medium replacement is lowered. Provided is a method for three-dimensionally culturing pluripotent stem cells wherein the pluripotent stem cells are cultured in a medium, said medium constantly containing 35 ng/mL or more of basic fibroblast growth factor, until the cell density attains 1.4×106 cells/mL or higher.
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