| abstract |
An isolated population of human pluripotent stem cells comprising at least 50% human pluripotent stem cells characterized by the expression signature of OCT4 + / TRA1-60 − / TRA1-81 − / SSEA1 + / SSEA4 − , and such population Are produced and maintained in suspension culture in a pluripotent undifferentiated state free of cell clumps. Also, a novel for culturing pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells in suspension culture or two-dimensional culture system while maintaining the cells in a proliferative and pluripotent undifferentiated state A culture medium is also provided. The new culture medium is interleukin 11 (IL11) and ciliary neurotrophic factor (CNTF); at least 50 ng / ml bFGF, and IL6RIL6 chimera; or animal-free contaminant-free serum replacement and IL6RIL6 chimera Including the body. Also, cell cultures containing pluripotent stem cells and culture media, and methods of using such cell cultures to expand and maintain pluripotent stem cells in an undifferentiated state, or lineage specific from pluripotent stem cells Also provided are methods of using such cell cultures to make cells. [Selection figure] None |