abstract |
The invention relates to a lateral flow diagnostic device and methods of use thereof. The device includes a substrate and a first end, wherein the first end includes a sample loading portion. The first end may further comprise a first detectable ligand-loaded region, a CRISPR effector system, a detection construct, a first test strip comprising a biotin ligand, and a second test strip comprising a capture molecule for the detectable ligand. The detection construct may comprise an RNA type oligonucleotide, having a first molecule such as FITC on a first end and a second molecule such as FAM on a second end. Contacting the sample loading portion with a sample causes the sample to flow from the sample loading portion of the substrate to the first and second capture regions, thereby generating a detectable signal, which may indicate a pathological condition. |