| abstract |
The invention discloses an artificial ventricle with a bionic spiral orientation microstructure and a preparation method thereof. The method comprises the following steps: s1, alternately extruding the myocardial fiber yarn carrying the myocardial cells and the hollow vascular fiber yarn carrying the angiogenesis cells by adopting a coaxial nozzle A and a coaxial nozzle B in the calcium ion cross-linking liquid, and collecting by a rotating ellipsoidal collector to realize 3D printing; meanwhile, the coaxial nozzles A and B are transversely moved in a reciprocating manner, and the deflection angle is changed layer by layer to obtain a fiber arrangement structure with spiral orientation; and S2, demolding from the ellipsoidal collector to obtain the artificial ventricle after the 3D printing is finished, and performing secondary crosslinking. The invention provides a new research model for researching myocardial regeneration and function regulation, the constructed heart physiology or pathology model is closer to the real state of a human body, the model is expected to replace an animal model in the aspects of heart disease research, drug screening and the like, and the invention has important guiding significance for developing a novel treatment strategy for repairing damaged myocardium. |