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filingDate 2017-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2018008309-A1
titleOfInvention Biological material quantifying method, image processing device, pathological diagnosis support system and program
abstract Provided are a biological material quantifying method, an image processing device, a pathological diagnosis support system and a program with which a specific biological material in a tissue specimen can be quantified accurately and simply. This biological material quantifying method is used to quantify a specific biological material in a sample that has been stained using fluorescent dye accumulating particles capable of binding to the specific biological material, and the method is characterized in being provided with: a step of extracting a certain region of a first fluorescence image obtained by capturing an image of the specimen, and calculating a first luminance integrated value by integrating luminance values of the certain region; and a particle count calculating step of calculating the number of fluorescent dye accumulating particles contained in a first region, from the first luminance integrated value and an average luminance value calculated from the distribution of a second luminance integrated value obtained by integrating the luminance values for each bright spot region, which indicate the emission of light by the fluorescent dye accumulating particles, in a second fluorescence image acquired by capturing an image of a preparation in which the fluorescent dye accumulating particles are dispersed without agglomerating.
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