| abstract |
The present invention provides: a method for increasing the yield, from a target monoclonal antibody, of a peptide fragment that includes an Fab region or variable region of the antibody or a portion of said region, said method being characterized by including a step in which the target monoclonal antibody, which is immobilized on the inner surface of pores in a porous body, and a protease, which is immobilized on the surface of nanoparticles having an average particle size that is larger than the average pore diameter of the porous body, are brought into contact in a liquid by stirring or tapping rotation stirring at a low speed of less than 100 rpm so as to maintain a uniform dispersion, and digestion of the target monoclonal body is carried out, wherein the average pore diameter of the porous body is in the range of 10-200 nm and the average particle size of the nanoparticles is in the range of 50-500 nm; and a kit to be used in said method. |