| abstract |
The present invention pertains to a peptide synthesis reaction using a carrier which can reversibly repeat a dissolved state and an encapsulated state, wherein it is easy to solve the problem of amino acid active species which are present in a reaction system during a deprotection reaction. The present invention (1) provides a peptide synthesis method containing the following steps: a step in which an N-Fmoc protected amino acid and a peptide having a C-terminus protected by a carrier is crystallized together with changes in the composition of a solvent in which the peptide is dissolved are condensated, in the presence of a condensing agent, to obtain an N-Fmoc-C-carrier protected peptide; a step in which a C1-14 alkyl amine or hydroxylamine is added to the reaction system; a step in which the N terminal is deprotected; and a separation step in which a solvent composition having a C-carrier protected peptide dissolved therein is varied, thereby crystallizing said peptide. |