| abstract |
An extended tissue fixation method is provided including at least one soak in a cold aldehyde-based fixative solution followed by a soak in a warm aldehyde-based fixative solution over a period greater than 2 days. Using the processes disclosed herein, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals, e.g. biomarkers, have been accomplished relative to standard room temperature fixation protocols. Moreover, the tissue can be left in the fixative solution up to at least 14 days using these methods, which provides improved flexibility relative to other protocols, enabling fixation to be conducted during transportation, shipping, and over weekends or vacations, while still achieving acceptable staining results. |