http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8460859-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d394e3a458d9c31ac77246f6aec69865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_35c349407fd1866558fcaa697fd5b353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3cde10605b30794fd676f517ea84dddf http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9abe3c5db943bda55e09f7baecde3962 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-00 |
filingDate | 2007-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d74e48bf3c7c74f10d4c355744f6065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d91111afeed0fde298e4b7bba077874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a378b8447d029b7060b43865002d28c0 |
publicationDate | 2013-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-8460859-B2 |
titleOfInvention | Tissue preservation and fixation method |
abstract | This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that is effective to fix the phosphoproteins, and that has a sufficient water content to be soluble for a stabilizer and/or a permeability enhancing agent); (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; and (3) a permeability enhancing agent (e.g. PEG). Methods are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. the following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10054521-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016120195-A1 |
priorityDate | 2006-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1491.