| abstract |
Provided herein are compositions aid methods for barcoding mammalian cells. The compositions and methods provided herein further provide methods for tracing such barcoded cells ex vivo or in vivo during the life time of an organism. In one aspect, a method of forming a barcoded cell is provided. The method includes expressing in a cell a heterologous cleaving protein complex including a sequence-specific DNA-binding domain and a nucleic acid cleaving domain. The sequence-specific DNA-binding domain targets the nucleic acid cleaving domain to a genomic nucleic acid sequence, thereby forming a genomic nucleic acid sequence bound to the heterologous cleaving protein complex. |