abstract |
The present invention provides a method for manufacturing a composite nanofiber support and a fish collagen/synthetic polymer nanofiber support manufactured by the method, wherein the method comprises: a first step for dissolving a synthetic polymer in an organic solvent; a second step for dissolving a fish collagen in water to prepare an aqueous fish collagen solution; a third step for adding the aqueous fish collagen solution prepared in the second step to the synthetic polymer solution prepared in the first step, followed by mixing; and a fourth step for electrospinning the mixture liquid prepared in the third step to manufacture a nanofiber support. In addition, the present invention provides a method for three-dimensional cell culturing using an agarose-collagen-alginate composite hydrogel support, and more specifically, to a method for three-dimensional cell culturing, the method comprising the steps of: mixing an alginate solution and cells, followed by culturing; adding a collagen solution to the cultured mixture to prevent the detachment of the cells; and adding an agarose solution to the collagen-added mixture, followed by standing and gelation. |