| abstract |
Methods and devices for conducting high-speed, high-resolution imaging of large intact tissue samples are provided. Aspects of the methods include placing a sample in an optically homogenous sample manipulation component, performing a calibration procedure to align a light sheet and a detection focal plane at a plurality of locations within the sample, and performing an imaging procedure on the sample to collect an image from each location. The collected images are reconstructed to form a three-dimensional image of the sample. Devices for carrying out the steps of the methods are also provided. |