| abstract |
The present invention relates to the field of biotechnology and medicine. More particularly, the invention concerns the production of DNA vectors with the inserted gene of a target protein and the production of recombinant protein in eukaryotic cell cultures. A composition for the intensive production of target protein in eukaryotic cells has been developed which comprises a DNA vector with inserted gene of a target protein and an agonist of cell receptors belonging to the pattern recognition receptor (PRR) family, which is selected from the following agonists: TLR2 agonists, or TLR4 agonists, or TLR5 agonists, or TLR7 agonists, or TLR8 agonists, or TLR9 agonists, or NODI receptor agonists, or NOD2 receptor agonists, used in optimal ratio. A composition for the intensive production of a target protein in eukaryotic cells may be also used, which comprises a DNA vector with the inserted gene of a target protein and an agonist of cell receptors belonging to the cytokine receptor family, used in optimal ratio. The methods for enhancing production of target protein in eukaryotic cell transduced with DNA vectors are used together with the above mentioned compositions. |