http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013081206-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a16f72268f5a1eb4f08073323a474668 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2011-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b046dabd890ef23bd19209b56b703e51 |
publicationDate | 2013-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2013081206-A1 |
titleOfInvention | Real-time pcr detection using stabilized probe |
abstract | Disclosed is a probe protector oligonucleotide for detecting an improved CATACLEAVE™ probe of a nucleic acid sequence from a test sample. The probe protector oligonucleotide is designed to be substantially mutually complementary to a FRET pair labeled CATACLEAVE™ oligonucleotide probe. A formation of a base pair between the probe protector oligonucleotide and a CATACLEAVE™ oligonucleotide probe that is combined to a chemical modification of a probe sequence prevents the formation of a spurious RNA:DNA hybrid during a PCR cycle, which can result in a non-specific cleavage of the oligonucleotide probe before the oligonucleotide probe is degraded by an endonuclease of an RNA sequence and before the detection of a target nucleic acid. An improved detection method is rapid and accurate and appropriate for high throughput application. Also disclosed is a kit, which is convenient, user-friendly, and reliable, for rapidly detecting a target nucleic acid sequence. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023026039-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2610380-A |
priorityDate | 2011-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.