| abstract |
A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a lysis section for lysing pathogens and leukocytes in the sample to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the pathogens and leukocytes in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the lysis section, the incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. |