| abstract |
A microfluidic system for isolation and amplification of DNA from aqueous solutions and detection of the DNA on a lateral flow detection strip, including a disposable microfluidic card for use in analysis of bacteria in platelets and an analysis of sexually transmitted diseases (STD) in urine. The card will include an embedded membrane that filters out cells and cellular debris. Any biological debris on the membrane will be lysed and the DNA amplified via PCR amplification protocol, including appropriate reagents and thermal cycling conditions. The amplified DNA is transferred to a lateral flow detection strip for a visual diagnostic read out. An alternate embodiment includes a microfluidic card for use in typing antiglobulin assays. |