Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5283edea7aac9ea8d39035f66db22f90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2eaeb616ce5f8dd45a1bc3f38cdb837e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d480586db94b6c1dee2495ca92ac23e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa5b41657881057e6ee019f8b3f89dbf http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_000ce3b7bfbb68c29c00640567e017fb |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
filingDate |
2009-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc4d79250afb3f6b4177f6d6b61759f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b96d5795aaf58fea4a49380eb18b7bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5aed7194f0f3e69b00aa947d5939d4fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f56e933e0e7a6ea1f019c60ff0088d88 |
publicationDate |
2010-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2010004736-A1 |
titleOfInvention |
Biosensor utilizing dna as element |
abstract |
Disclosed is a method which can detect and/or quantify an analyte in a sample by utilizing a sensor protein that can bind to the analyte specifically and a nucleic acid that can be recognized by the sensor protein specifically, and which is excellent in terms of cost, operability and rapidity. ArsR protein (which is a sensor protein capable of binding to arsenic) and green fluorescent protein (GFP) are fused together to produce ArsR-GFP. It is confirmed that this fusion protein can bind to a specific recognition sequence (Pars-DNA), and it is also confirmed that the binding between the fusion protein and Pars-DNA can be inhibited by arsenious acid. Next, a plate having Pars-DNA immobilized thereon is prepared. It is found that the quantity of ArsR-GFP bound to the Pars-DNA-immobilized plate is decreased in an arsenious acid concentration-dependent manner. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2972054-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9040240-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012114046-A1 |
priorityDate |
2008-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |