| abstract |
The present invention provides a method for preparing iPS cells without use of such a vector that causes the integration of a foreign gene into the chromosomes of a host cell, for example, a retrovirus vector or a lentivirus vector, and a kit for preparing the same. The method for preparing iPS cells comprises a nuclear reprogramming factor introduction step which involves introducing, into somatic cells, an episomal vector into which the gene encoding a nuclear reprogramming factor is inserted in an expressible form; a cultivation step which involves culturing somatic cells having the episomal vector introduced therein; and a selection step which involves selecting iPS cells generated by reprogramming of the somatic cells. |