| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_829870c3d1719dc25f6939097d79a0e3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff12915e4d1ce5442d479bd5a88d2e70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c588997ee7d46fd58c65eef5ed0ed61a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_addf9108e0141eb652310061b340b9da |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac8a13c89765b64d8a5f29a2e6eb5ea7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_965c6b487897cd2df51b07b33dfbbca3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0873744602e199e00bf13adb4d18cb18 |
| publicationDate |
2009-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009082190-A1 |
| titleOfInvention |
An improved mutagenesis method using polyethylene glycol mediated introduction of mutagenic nucleobases into plant protoplasts |
| abstract |
Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/IL-263595-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8268622-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016105185-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019193143-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017222370-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11371051-B2 |
| priorityDate |
2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |